Flash Cards
Simply click the image to flip over the flash cards below.
You can use these as a way to test your understanding of the material and to prepare for the Biotechnology Identification Contest if you are an FFA student.
Flash Cards
Page 1 of 3
What is this piece of equipment?
Electrophoresis casting tray
The mold into which the hot agarose liquid is poured.
Photo Source 2: Joseph Elsbernd
What is this instrument?
Micro-pipet
A micro-pipet is a tool used in a laboratory to transfer a measured volume of liquid. A micro-pipet is often used when filling wells in an electrophoresis gel.
Photo Source 3: Public Domain
What are the scientists doing in this photo?
Loading a gel
In gel electrophoresis, DNA samples are loaded into slots in agarose gel, known as wells, using a micro-pipet. The DNA samples are colorless, but a blue tracking dye can be added to the sample so that the DNA can be visually tracked as it moves through the gel.
Photo Source 7: Blaz Nemec
What piece of equipment is in this photo?
Centrifuge
In the centrifuge, an object is put in rotation around a fixed axis, applying a force perpendicular to the axis. The centrifuge is used to separate material from the mediums they are mixed with. In a DNA lab, a centrifuge can be used in DNA extraction.
Photo Source 16: Magnus Manske
What part of the cell is the red arrow pointing to?
Nucleus
The nucleus is a membrane bound structure that contains DNA and RNA – the cell’s hereditary information. It controls the cell’s growth and reproduction.
Photo Source 9: Dr. Don Lee, UNL
What is depicted in this graphic?
Chromosomes
Chromosomes are thread-like structures found in the nucleus of the cell. Each chromosome contains DNA, protein, and RNA.
Photo Source 12: Public Domain
What are the items shown here?
Micro-centrifuge tubes
Micro-centrifuge small tubes are used to store and centrifuge small amounts of liquid.
Photo Source 10: Zephyris
What is the circle indicating in this photo?
Agarose gel
Agarose gel, also called Agar gel, is used as a medium for growing cultures and used to conduct gel electrophoresis tests.
Photo Source 7: Blaz Nemec
What is the long white pieces shown in these photos?
Protein Test Strips
Protein test strips are used to detect the presence of proteins in a substance. It is most frequently used to detect the presence of GMOs.
Photo Source 6: Teststrips
What equipment is the arrow pointing to?
Incubator
An incubator is used to grow and maintain microbiological cultures or cell cultures.
Who works with this?
The plant transformation specialist uses this to grow colonies of Agrobacterium. She then uses the Agrobacterium to deliver the transgene to plant cells.
Photo Source 5: : Matylda Sęk
Who works with this?
It’s the job of the transgene designer to identify these gene regions and put them together.
Image Source 24: Dr. Don Lee – UNL (24)
This tool allows transformation scientists to insert transgenes into embryos
High-powered microscope
A sequence of DNA found on a chromosome that provides genetic instructions to make a protein
Gene
Genes are found on these large molecules
Chromosomes
This organism is used to provide a coding region for the transgenic pigs in Enviropig
E. Coli
The instruction manual for a cell
DNA
The name of the method used for inserting a transgene into an embryo
Pronuclear Injection
A visual depiction of all the possible ways the alleles from two parents could combine.
Punnett Square
A diagram that shows how traits are passed down through generations
Pedigree
An individual with two of the same allele
Homozygote
An individual that has two different forms of a gene (alleles)
Heterozygote
when many copies of the same DNA fragment move together through an electrophoresis gel it creates this
Band
A method for making lots of copies of a particular gene or sequence of DNA in the lab
Polymerase Chain Reaction (PCR)